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Damini Singh, Speaker at Vaccines Conferences
Environmental Pollution Analysis Lab, India
Title : Micro encapsulations of Outer membrane proteins of Brucella Spp. highly potent vaccines against brucellosis


Brucellosis is a zoonotic bacterial infection of pandemic potential – caused by Brucella species. Lack of vaccines against human brucellosis despite continuous research has led us to address this issue using several selectively chosen antigens pertaining to Brucella spp. We have been utilizing different marketed adjuvants e.g. Aluminium hydroxide, MF-59 etc. Simultaneously, we have been developing nano-encapsulations of various antigens namely – rL7/L12, rOmp25 and rOmp28 etc.

Poly-lactide-co-glycolide (PLGA) has been one pf the most successful with all the antigens mentioned above in eliciting a good humoral immune response as well as causing a huge reduction in the organs’ bacterial load especially liver and spleen. Additionally, we have been using nano-liposomes which were similar in their efficiacy aspects but had much inferior stability upon storage. 

Post-immunization, several parameters e.g. IgG antibody levels, cytokines – their levels, and bacterial load in the organs and all the antigens being highly immunodominant produced significantly high antibody titers apart from ensuing varied cytokine release patterns.

To summarise, Omps were found to spike apical immune responses as well as maximal protective efficacy. Thus, Omps hold a huge promise as prophylactics of future use. More research is aimed to be conducted to understand the immune memory pertaining to these antigenic formulations.


Damini Singh finished her Doctoral studies from Jawaharlal Nehru University, New Delhi, India - this was focussed at the Development of a Brucella Vaccine suitable for humans. Biodegradable Nano and microparticles encapsulated with conserved as well as immunodominant antigens of Brucella spp. An extension study in this context, to receive a "Young scientist award" by Department of Health Research, Government of India. Further, for post-doctoral research, She had joined "W.M.Keck Centre for Transgene research" at University of NotreDame, Indiana, U.S.A wherein the research was mainly aimed at studying the Group A Streptococci, it's virulenece in mice - wild type as well as knock outs of various blood proteins. Currently, She is  working as Scientist at Environmental Pollution Analysis Lab - inculdes protocols' optimization and preparation of materials for trainings, workshops etc as well as preparation of research projects.