Title : Silver and gold bimetallic nanoparticles as an innovative bacterial vaccine platform against infections caused by ESKAPE group species
Abstract:
Outer Membrane Vesicles (OMV) is an innovative vaccine technology against bacterial infections. The OMVs are proposed as a vaccine platform for the presence of outer membrane proteins that act as antigens and PAMPs. Silver and gold nanoparticles provide a robust immune system response against bacterial infections by blocking receptor-virus interaction, improving anti-inflammatory activity, promoting opsonophagocytosis by macrophages and increasing CD4 and CD8 T cells levels. Thus, the main goal of this project is to develop a Ag@AuNPs vaccine platform with OMV as a preventive alternative for infectious diseases by the ESKAPE group.
Ag@AuNPs will be synthesized by extract of plants and combined with outer membrane vesicles from Gram-negative bacterias. The biophysical characterization will be performed to evaluate the properties of Ag@AuNPs coated with vesicles. The immunogenicity of Ag@AuNPs vesicles will be put to test through immunological assays using murine models.
The biophysical features of ESKAPE-Ag@AuNPs vaccine, such as, size distribution and zeta potential measurements provided by Dynamic Light Scattering and the visualization of spherical Ag@AuNPs by transmission electron microscopy are expected to be found. Such as, significant increase of IgG titers in a post-vax rabbit serum, improvement of ESKAPE killing by neutrophils in post-vax serum, as well as, inducement of robust B cell recruitment and DC activation by nanoparticle vaccines.
In conclusion, the vaccine developed with nanoparticles has been seen as an innovative therapeutic alternative to avoid infection caused by multi-resistant bacterias, due to their antimicrobial and immunomodulatory properties, leading to antimicrobial resistance decrease.
