Title : Comparison between virulent genes-based and serotype-based vaccine for uropathogenic escherichia coli
Abstract:
Urinary tract infections (UTIs) are one of the most common bacterial infections with global expansion particularly in Egypt. These infections are predominantly caused by uropathogenic Escherichia coli (UPEC). In a trial to prevent these infection we proposed this study in which monovalent verses polyvalent vaccines were evaluated after the presence of specific bacterial genes that encode virulence factors was determined. The virulent genes explored include ibeA, pap, sfa/foc, cnf1, hly, fyuA, pil, ompT and traT. Mice were vaccinated with whole cell or cell free formaline-killed selected strain of E.coli (O78 serotype) as monovlanent vaccine. The polyvalent one was a combination of three other selected strains, serotype O78, O114 and O164, each has different virulent genes content. Testing the efficiency of vacines with and without adjuvant was also considered. We assessed the efficacy of vaccination using ten mouse for each treatment. Depending on data obtained the vaccine preparation with a high immune response that gave high protective index and elevated antibody level, was the polyvalent whole cell formalin-killed vaccine combined with adjuvant. This finding suggests that this polyvalent vaccine may has utility in preventing UTIs in humans regardless of the virulent gene content of the strain causes infection.
Audience Take Away:
- This paper benefit all vaccine developers
- Immunologists and vaccinologists also need it
- Microbiologists of medical interest are involved by paper results
- The old methods still good for vaccine strains selection
- The paper focus on the serology- based selection is better than Molecular one
