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Jamil M A S Obaid, Speaker at Vaccines Conferences
Ibb University, Yemen
Title : Comparison between virulent genes-based and serotype-based vaccine for uropathogenic escherichia coli


Urinary tract infections (UTIs) are one of the most common bacterial infections with global expansion particularly in Egypt. These infections are predominantly caused by uropathogenic Escherichia coli (UPEC). In a trial to prevent these infection we proposed this study in which monovalent verses polyvalent vaccines were evaluated after the presence of specific bacterial genes that encode virulence factors was determined. The virulent genes explored include ibeA, pap, sfa/foc, cnf1, hly, fyuA, pil, ompT and traT. Mice were vaccinated with whole cell or cell free formaline-killed selected strain of E.coli (O78 serotype) as monovlanent vaccine. The polyvalent one was a combination of three other selected strains, serotype O78, O114 and O164, each has different virulent genes content. Testing the efficiency of vacines with and without adjuvant was also considered. We assessed the efficacy of vaccination using ten mouse for each treatment. Depending on data obtained the vaccine preparation with a high immune response that gave high protective index and elevated antibody level, was the polyvalent whole cell formalin-killed vaccine combined with adjuvant. This finding suggests that this  polyvalent vaccine may has utility in preventing UTIs in humans regardless of the virulent gene content of the strain causes infection.

Audience Take Away:

  • This paper benefit all vaccine developers
  • Immunologists and vaccinologists also need it
  • Microbiologists of medical interest are involved by paper results
  • The old methods still good for vaccine strains selection
  • The paper focus on the serology- based selection is better than Molecular one


Dr. Jamil M.A.S. Obaid is an Assistant professor of immunology and immunohematology, Head of Medical laboratory Sciences department at the Faculty of Medicine and Health Sciences, Ibb University since 2016. He is lecturer at the Medical Microbiology department at the Faculty of Science and many private universities for medical and health sciences students in the field of immunology, immunohematology, hematology, vaccinology and Clinical Laboratory sciences. He is lecturer for Master degree students, examiner and supervise for two students. He have 11 research articles and many studies under publication.