HYBRID EVENT: You can participate in person at Boston, Massachusetts, USA or Virtually from your home or work.
Christine Bricault, Speaker at Vaccines Conferences
Sanofi Vaccines, United States
Title : Characterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus from two older adults

Abstract:

Human metapneumovirus (hMPV) is a major cause of acute respiratory tract infections in both infants and older adults. Currently there are no approved vaccines or antibody therapies for treatment against infection from hMPV. The key target of neutralizing antibodies is the viral fusion (F) glycoprotein, which is required for viral entry. However, little is known about the humoral immune response elicited by humans after natural infection with hMPV. In this study, we used stabilized hMPV F protein trimers to isolate and sort hMPV F-specific B cells from two older adults. We obtained hundreds of class-switched antibody sequences representing over 500 clonotypes, indicative of a highly polyclonal antibody response to hMPV F in these two individuals. In-depth characterization of 136 of these monoclonal antibodies revealed broad recognition of the hMPV F surface, with potent neutralizing antibodies targeting several distinct antigenic sites. Cryo-EM structures were solved for two of the neutralizing antibodies in complex with hMPV F, revealing the molecular basis for recognition of two prefusion-specific epitopes at the apex of the F trimer. These results provide new insights into the humoral response to hMPV infection in older adults and can help guide development of novel vaccine immunogens.

Audience Take Away:

  • How hMPV F-specific antibodies from human PBMC were isolated
  • Characteristics of antibodies to distinct epitopes on the surface of hMPV F trimer
    Information that could be used for improved hMPV F immunogen design

Biography:

Dr. Bricault studied Biology at Cornell University and graduated with a B.S. in 2010. She then joined the research group of Professor Dan Barouch at Harvard University in the Center for Virology and Vaccine Research where she completed her Ph.D. in Virology in 2018 and then completed a two-year postdoctoral fellowship. She then joined Sanofi Vaccines in 2018 and has been working on antibody discovery and single cell technologies efforts.

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